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An AST file is an audio file used in a Nintendo Wii video game. It contains in-game music. Most notably, Super Mario Galaxy 1 and 2 and The Legend of Zelda: Twilight Princess use AST files.

The AST file format is one of many proprietary file formats Nintendo uses to store in-game music. Those who rip the contents of their Wii game discs or explore Wii-related forums are most likely to encounter AST files, extracted from certain Wii video games. A variety of Super Mario Galaxy's AST files, for example, can be found online.

You can open an AST file with vgmstream (multiplatform), to play the music it contains. You can also play AST files with Foobar2000 or Nullsoft Winamp, after installing those programs' vgmstream plug-ins.

You can use astConverter (Windows) to convert AST files to the .MP3, .WAV, or .OGG formats. You can also use Foobar2000 to convert AST files to WAV and other formats, provided you have the vgmstream plug-in installed.

Spreadsheet template created by Ability Spreadsheet, an application that is part of the productivity suite Ability Office; used for creating multiple Ability spreadsheets (.AWS files) from the same template.

Contains color separation options that can be loaded by several Adobe programs; may be used for defining color separations for printing, exporting to PDF, or exporting to another program; can be used for images using RGB, CMYK, and other color modes.

Another common color separations format is a "Desktop Color Separations" (.DCS) file, which was developed by Quark.