.NDPI File Extension

Hamamatsu TIFF-like Slide Image

Developer Hamamatsu
2.7  |  3 Votes

What is an NDPI file?

An NDPI file is a pathology slide specimen image created by a Hamamatsu slide scanner, such as the Hamamatsu NanoZoomer. It contains a picture of a slide specimen, saved in a proprietary, TIFF-like format. NDPI files can be viewed in Hamamatsu's slide-viewing software or in other slide-viewing applications.

More Information

Pathologists use virtual microscopes and digital slide scanners to save and examine digital images of pathology slide specimens. Some Hamamatsu slide scanners save slide images as NDPI files. These files are TIFF-like files that contain .JPEG images and metadata that specifies the images' size, intended resolution, and other data.

However, because of the way NDPI files are structured, they cannot be read as normal TIFF files. For example, NDPI files' headers are not structured the same way as standard TIFF headers, which can cause applications that can open TIFF files to fail to open NDPI files. Only certain slide-viewing applications allow users to view NDPI files.

How to open an NDPI file

You can open NDPI files in Aperio Technologies ImageScope (Windows), NetScope Viewer (Windows), NDPITools (cross-platform), or OpenSlide (cross-platform).

Open over 400 file formats with File Viewer Plus.Free Download

Programs that open NDPI files

Verified by FileInfo.com

The FileInfo.com team has independently researched the Hamamatsu TIFF-like Slide Image file format and Mac, Windows, and Linux apps listed on this page. Our goal is 100% accuracy and we only publish information about file types that we have verified.

If you would like to suggest any additions or updates to this page, please let us know.